Description

High density lipoproteins (HDLs) high levels are associated with a reduction of the cardiovascular risks, related to an inhibition of vascular inflammation and to antioxidant and antithrombotic properties. Different subpopulations and subfractions of HDLs are known but the functionality of individual HDL subpopulation is not well documented at this stage, due to their high heterogeneity and the continual interconversion from a subpopulation to another in the plasma. HDLs are micro-RNAs (miRNAs) carrier. miRNAs are small, endogenous non-coding RNAs which have an important post-transcriptional function. In this project, we determined the relationship between miRNA-associated HDLs transcoronary gradient and changes in HDLs composition and structure in the aim to predict the stable or unstable nature of Coronary Artery Disease (CAD). In particular, we isolated HDLs from patients healthiest or suffering of cardiovascular disease (Stable angina, acute coronary syndrome (ACS)) and described in blood from the coronary sinus, an artery and a peripheral vein. HDL composition and structure and miRNAs-associated HDL concentrations were measured. We observed there was no transcoronary gradient of HDL main components and there was no relationship between miRNAs HDL associated and HDLs composition and structure. Nevertheless, we determined than HDL-associated miRNAs can predict the stable vs. unstable nature of the disease and could be used as biomarkers.

Labs involved

• Centre for Vascular Research, The University of New South Wales 2052, Sydney, Australia

Publications

• Choteau SA, Cuesta Torres LF, Barraclough JY, Elder AMM, Martínez GJ, Chen Fan WY, Shrestha S, Ong KL, Barter PJ, Celermajer DS, Rye KA, Patel S, Tabet F (2018). Transcoronary gradients of HDL-associated microRNAs in unstable coronary artery. International Journal of Cardiology. 253:138144 doi:10.1016/j.card.2017.09.190.
• Tabet F, Cuesta Torres LF, Ong KL, Shrestha S, Choteau SA, Barter PJ, Clifton P, Rye KA (2016). High-Density Lipoprotein-associated miR-223 is altered after diet-induced weight loss in overweight and obese males. PLoS One. 11(3):e0151061 doi:10.1371/journal.pone.0151061.

Close collaborators

• Fatiha Tabet
• Philip J Barter
• Kerry-Anne Rye
• Luisa F Cuesta Torres
• Sudichhya Shrestha
• Kwok L Ong