References available on request

2017 (6 months): Sanofi-Aventis R&D, Marcy l’Etoile (France)
Division of Infectious Diseases, Microbe – Omics team
Confidential work
Microbiology, biochemistry, bacteriology, infectiology
Supervised by L. Conraux. Team of A. Vassort. Followed and evaluated by S. D’Andréa (AgroParisTech)



2015 – 2016 (5 months): University of New South Wales, Sydney (Australia)
Lipid Research Group, School of Medical Sciences, Faculty of Medicine
Lipidology, biochemistry, human nutrition, cardiology
Cardiovascular diseases (atherosclerosis) and HDLs (high-density lipoproteins)
Supervised by F. Tabet. Team of K-A Rye and P.J. Barter. Followed and evaluated by V. Rioux (Agrocampus Ouest, Laboratory of Nutrition and Biochemistry)
Characterization of High Density Lipoproteins (HDLs) composition and structure in different bloods from patient with cardiovascular diseases (Acute Coronary Syndrom / Stable angina) and controls
Characterization of HDLs composition in blood from patients under low-fat high protein diet and patients low-fat high carbohydrate diet before and after a diet
Laboratory work
> HDL extraction from plasma by immunoprecipitation (apoA-I antibodies)
> Non-apoB fraction extraction from plasma by PEG precipitation
> Highlight of HDLs subpopulation by non-denaturing Gradient Gel Electrophoresis
> Measure of HDL main component concentrations (Phospholipids, free cholesterol, total cholesterol, triglycerides, apoA-I, apoA-II, apoB, total proteins) using an auto-analyser (AU480 Beckman Coulter)
> Measure of total proteins concentration by BCA assay (spectrophotometry on plates, Omega plate reader)
> miRNA extraction – Reverse-transcription and TaqMan – quantitative Polymerase Chain Reactions

– Worked in autonomy in a PC2 laboratory

2 publications


2015 (1 month): Work placement in a farm – EARL de l’Enclos Rouge, M. Bardé, Vacqueriette-Erquières (France)
Technical work (bovine breeding and polyculture)
– Farm management analysis (management of productions, economical analysis of the company…)
– Discussion about genetics as tool in farm management