Internships

References available on request

2017 (6 months): Sanofi-Aventis R&D, Marcy l’Etoile (France)
Division of Infectious Diseases, Microbe – Omics team
Confidential work
Microbiology, biochemistry, bacteriology, infectiology
Supervised by L. Conraux. Team of A. Vassort. Followed and evaluated by S. D’Andréa (AgroParisTech)

   

 

2015 – 2016 (5 months): University of New South Wales, Sydney (Australia)
Lipid Research Group, School of Medical Sciences, Faculty of Medicine
Lipidology, biochemistry, human nutrition, cardiology
Cardiovascular diseases (atherosclerosis) and HDLs (high-density lipoproteins)
Supervised by F. Tabet. Team of K-A Rye and P.J. Barter. Followed and evaluated by V. Rioux (Agrocampus Ouest, Laboratory of Nutrition and Biochemistry)
Projects:
Characterization of High Density Lipoproteins (HDLs) composition and structure in different bloods from patient with cardiovascular diseases (Acute Coronary Syndrom / Stable angina) and controls
Characterization of HDLs composition in blood from patients under low-fat high protein diet and patients low-fat high carbohydrate diet before and after a diet
Laboratory work
> HDL extraction from plasma by immunoprecipitation (apoA-I antibodies)
> Non-apoB fraction extraction from plasma by PEG precipitation
> Highlight of HDLs subpopulation by non-denaturing Gradient Gel Electrophoresis
> Measure of HDL main component concentrations (Phospholipids, free cholesterol, total cholesterol, triglycerides, apoA-I, apoA-II, apoB, total proteins) using an auto-analyser (AU480 Beckman Coulter)
> Measure of total proteins concentration by BCA assay (spectrophotometry on plates, Omega plate reader)
> miRNA extraction – Reverse-transcription and TaqMan – quantitative Polymerase Chain Reactions

– Worked in autonomy in a PC2 laboratory

2 publications

 

2015 (1 month): Work placement in a farm – EARL de l’Enclos Rouge, M. Bardé, Vacqueriette-Erquières (France)
Technical work (bovine breeding and polyculture)
– Farm management analysis (management of productions, economical analysis of the company…)
– Discussion about genetics as tool in farm management