Experimental skills

Experimental skills

My education and professional experiences gave me the opportunity to develop several experimental skills. You could find here a (non-exhaustive) list of these skills.
Please, feel free to contact me if you wish to have more information about my skills or about the context in which I developed these skills.
I’ll be happy to have a discussion with you.

 

    General skills ****

  • Cellular culture **
  • Bacterial culture (on liquid and solid media) ***
  • Manipulation of liquid nitrogen ***
  • Work in P2 laboratories (manipulation of GMOs, human samples and infectious agents) ****
  • Preparation of samples, buffers, media and reagents … ****

 

    Biochemistry, analytical chemistry and organic chemistry ***

  • Proteins, lipids, lipoproteins extraction and purification (from mammalian cells and bacteria) ****
    • Immunoprecipitation ****
    • Polyethylene glycol (PEG) precipitation ****
    • Tissue homogenization / cell lysis ****
  • Total proteins quantification (BCA assay, Bradford assay) ****
  • Chromatography
    • Affinity chromatography **
    • Thin Layer Chromatography (TLC)***
    • Size-exclusion chromatography (SEC)**
    • High Performance Liquid chromatography (HPLC) **
    • Gas Chromatography **
  • Electrophoresis
    • Sodium / Lithium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE / LDS-PAGE) ****
    • Gradient Gel Electrophoresis (GGE) ****
    • Capillar electrophoresis **
  • Western-blot ****
  • Spectrometric assays
    • UV spectrophotometry ****
    • IR spectrophotometry ***
    • Mass spectrometry (MS) *
  • Turbidimetric assays ***
  • Enzyme-linked immunosorbent assays (ELISA) **
  • Steam and water distillation ***

 

    Microbiology ***

  • Bacterial culture and pathogen manipulation ***
  • Microorganism identfication
    • Macroscopic and microscpic description **
    • Gram coloration, oxidase catalase assays ***
    • Metabolic profiles: API **
  • Microbial cell activity assays ***
  • CFU determination ****
  • Genetic engineering and cloning
    • Microbial transfection, preparation of competent bacteria ***
    • Gateway cloning system **
    • Restriction-ligature cloning system **
    • Design of plasmid construction **
  • Fermentation driving *

 

    Genetics ***

  • Plasmid extraction and purification ***
  • RNA, miRNA extraction ***
  • Reverse-transcriptase PCR (RT-PCR) **
  • Real-time PCR TaqMan (qPCR) **

 

    Imaging and microfluidics *

  • Microscopy
    • Optical microscopy ***
    • Confocal microscopy *
  • Flow cytometry *

 

    Used to work (in autonomy) with the following material

  • Auto-analyzer AU480 Beckman Coulter (use and maintenance) ****
  • Plate readers (Enspire Perkin Elmer, FLUOstar Omega BMG Labtech …) ****
  • Centrifuges ****
  • Thermo-cyclers (Bio-rad, Applied Biosystems …)****
  • Spectrophotometers (NanoDrop …)****
  • Imaging systems for SDS-PAGE and Western-blot (Odyssey …)****
  • Tissue homogenizer (Precellys Bertin technologies, Cryolis, FastPrep) ****
  • Microbiological incubators and environmental chambers****
  • Eddy jet spiral plater ****
  • Gas chromatograph – Mass spectrometer *
  • Fermenter (Statorius Biostat A+) **